Characterization of Physicochemical Properties of Melanin Produced by Gluconobacter oxydans FBFS 97

The present study aimed to characterize melanin pigment extracted from Gluconobacter oxydans FBFS 97. After 14 days of culture at 28 °C in GY (glucose and yeast extract) liquid-state medium, G. FBFS97 produce the maximum melanin, up about 12–15 mg/L. physicochemical characteristics showed an ability dissolve 1 mol/L NaOH or KOH, insolubility water most organic solvents, such as chloroform petroleum ether. was confirmed be exact by ultraviolet-visible spectrophotometry, Fourier-transform infrared spectroscopy, thin-layer chromatography, elemental analysis, scanning electron microscopy. UV-visible spectrum exhibited a absorption peak 230 nm. Extracted demonstrated significant free radical-scavenging activity DPPH ABTS methods. IC50 value for scavenging 50% radicals 36.94 ?g/mL, antioxidant 4.06 ?g/mL. Hence, has potential new candidate production.